NH4Ac/EtOH ppt of DNA from PCR ?
Dr. Duncan Clark
duncan at genesys.demon.co.uk
Fri Mar 8 06:03:52 EST 1996
In article <4hnthh$815 at metro.usyd.edu.au>, Royal Botanic Gardens
<rbgs00 at angis.su.oz.au> writes
>From: rbgs00 at angis.su.oz.au (Royal Botanic Gardens)
>Path: angis.su.oz.au!rbgs00
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: NH4Ac/EtOH ppt of DNA from PCRs ??
>Expires:
>References:
>Sender:
>Reply-To: rbgs00 at angis.su.oz.au (Royal Botanic Gardens)
>Followup-To:
>Distribution:
>Organization: ANGIS, The University of Sydney, Australia
>Keywords:
>
>Hi folks
>
>Could someone please give me a recipe for differential precipitation
>of DNA (without oligos and dNTPs) from PCRs, using ammonium acetate
>and ethanol?
>
See Wayne Barnes, Gene Vol 112 pp 29-35 (1992).
Duncan
DNAmp Ltd.
Licensed PCR enzyme manufacturer
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