NH4Ac/EtOH ppt of DNA from PCR ?

Dr. Duncan Clark duncan at genesys.demon.co.uk
Fri Mar 8 06:03:52 EST 1996


In article <4hnthh$815 at metro.usyd.edu.au>, Royal Botanic Gardens
<rbgs00 at angis.su.oz.au> writes
>From: rbgs00 at angis.su.oz.au (Royal Botanic Gardens)
>Path: angis.su.oz.au!rbgs00
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: NH4Ac/EtOH ppt of DNA from PCRs ??
>Expires: 
>References: 
>Sender: 
>Reply-To: rbgs00 at angis.su.oz.au (Royal Botanic Gardens)
>Followup-To: 
>Distribution: 
>Organization: ANGIS, The University of Sydney, Australia
>Keywords: 
>
>Hi folks
>
>Could someone please give me a recipe for differential precipitation
>of DNA (without oligos and dNTPs) from PCRs, using ammonium acetate
>and ethanol?
>

See Wayne Barnes, Gene Vol 112 pp 29-35 (1992).

Duncan 
DNAmp Ltd.
Licensed PCR enzyme manufacturer
--------------------------------------------------------------------------------
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....
http://www.thenet.co.uk/~dnamp



More information about the Methods mailing list