HELP!---Immunoaffinity column doesn't work

Jan Schroeder jschroed at vzdmza.zdv.uni-mainz.de
Wed Mar 6 06:20:54 EST 1996


Hi there,

I am quite new to handle with Immunoaffinity Chromatography and I want to purify a protein from Hordeum vulgare with this subject.
Before I start to purify the protein. I have to clean the polyclonal mouse IgG with a protein A column (BioRad, Affi-Gel Protein A Gel). The antibody did not bound to the protein A and I found the complete IgG in the first wash step. Then I tried a different way:
I tried to couple the IgG via caboanhydrate moities to hydrazide activated agarose beats (BioRad, Affi-Gel Hz Immunoaffinity Column) In the first step I detected again the complete IgG so it did not bind to the gel.
Maybe there are eome hints behind the productinformations?
Please contact Jan Schroeder<jschroed at vzdmza.zdv.uni-mainz.de>
Thanks, Tanja



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