bhassel at orion.rz.mdc-berlin.de
Fri Mar 8 10:33:54 EST 1996
In article <4guumc$p9l at osfb.aber.ac.uk>
jms93 at aber.ac.uk (jms93) writes:
> I have noticed that if I change the quantity of
> >the first strand cDNA into the PCR reaction that the intensity of the
> >band changes. Amazingly, the less the quatity of the cDNA the stronger
> >the band up to a certain threshold.
Maybe, if there is too much of EDTA in your (stopped) first strand reaction, it
can complex the Mg++ in your PCR-buffer to a suboptimal concentration.
Just an idea.
Burkhard Hassel (bhassel at orion.rz.mdc-berlin.de)
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