RT-PCR

[Burkhard Hassel]

In article <4guumc$p9l at osfb.aber.ac.uk>
jms93 at aber.ac.uk (jms93) writes:

> I have noticed that if I change the quantity of 
> >the first strand cDNA into the PCR reaction that the intensity of the 
> >band changes. Amazingly, the less the quatity of the cDNA the stronger 
> >the band up to a certain threshold. 

Maybe, if there is too much of EDTA in your (stopped) first strand reaction, it
can complex the Mg++ in your PCR-buffer to a suboptimal concentration.
Just an idea.

Burkhard Hassel (bhassel at orion.rz.mdc-berlin.de)