Sequencing Gel Rigs for SSCP

[WSchick at aol.com]

In a message dated 96-03-08 07:53:15 EST, ai80 at dial.pipex.com (Dr G R Taylor)
writes:

>
>I'm trying to source a sequencing length gel rig (i.e. 36cm long or 
>longer) for SSCP work.  Rather than run one in a cold room,  I would like 
>one that has built-in cooling.  So far the only one I've seen is the 
>Pharmacia system,  which is good,  but pricey.  Does anyone know of any 
>alternatives?

Hoefer/Pharmacia also has the SE660 and SE620  which are long protein type
gel units, fully cooled on both sides of the sandwiches.  They run one or two
gels at a time, are 24 inches and 32 inches.   A recent paper by someone at
NIH has used these with thinner spacers for SSCP.  Prices are a little over
$1000.   With this unit you could also change the lower buffer chamber to run
shorter gels for DGGE, proteins, and vertical agarose.
You can probably get a demo of this unit, and the local Hoefer Pharmacia rep
should lhave reprints of relevant papers.

I used to work at Hoefer, but have no commercial ties.  I do own 50 shares of
Pharmacia, however!

Walter Schick
Biotech research marketing and sales
Bio-Sep Co.
872 Autumn Dr.
Walnut Creek, CA 94598 USA
PH/Fax +1-510-945-6360
e-mail wschick at aol.com