Wizard PCR purification from agarose gel

ruth e.w. walker pharmrew at muccmail.missouri.edu
Fri Mar 8 15:19:34 EST 1996


I am unable to purify my PCR product from the gel using the Wizard kit. 
It works well when I purify directly from the PCR rxn. I use 2% agarose, 
which melts within 4 min at 65C in presence of the resin. Is that too 
long? Too short? What else could be wrong, given that it does work with 
the PCR rxn, done concurrently?




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