Taq stability?

Christoph Ahlers CAH at ifm.mh-hannover.de
Sat Mar 9 05:17:01 EST 1996


In article <4hn278$ni1 at taco.cc.ncsu.edu>, Susan Jane Hogarth
<sjhogart at unity.ncsu.edu> wrote:

> Maybe this has been addressed before, but I've been wondering...
> 
> If Taq polymerase is so heat-stable that it can function after 40+
rounds of 95
> degrees, why are people so uptight about letting it get out of the freezer for
> more than 3.5 seconds?
> -- 

The local Perkin-Elmer distributor ships their Taq at room temp., and I've
also heard stories of salesmen transporting their Taq products/samples in
the car without refrigeration.  I think short-term it's ok at RT, for
storage tho I keep mine at -20.

Chris

-- 
Chris Ahlers
Institute for Molecular Biology
Hannover Medical School
30625 Hannover, Germany
Tel. 49 511 532 5956
Fax  49 511 532 4283
cah at ifm.mh-hannover.de



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