Polyacrylamide gel fixation for DNA
buzardg at MAIL.NCIFCRF.GOV
Sun Mar 10 22:27:30 EST 1996
At 03:39 PM 3/10/96 -0700, you wrote:
>Thanks for the information.
>I will consider Sybr Green, especially as a stain of sequencing gels.
>That would be quite a trick. How do you preserve the image for analysis?
The image is photographed with a special wavelength filter, although the
E.Br. filter will function, on a Polaroid or Imaging system. You need a
system that moves, or reduces the image, so the fixed-hood cameras are
useless, almost. Another trick of Dick Calvert has been to place a
completely UV-transparent plastic wrap tightly over the drained film after
staining and flipping the plate over for exposure to the UV. If you are
fortunate to have one of the old indirect overhead UV lamp systems gathering
dust in the closet, it is even more convenient.
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