Distinct bands in restriction dig. of genomic DNA

ae at dna.bio.warwick.ac.uk ae at dna.bio.warwick.ac.uk
Sun Mar 10 11:34:35 EST 1996


In article <mantei-1003961115330001 at retrograde.ethz.ch> mantei at neuro.biol.ethz.ch (Ned Mantei) writes:
>From: mantei at neuro.biol.ethz.ch (Ned Mantei)
>Subject: Re: Distinct bands in restriction dig. of genomic DNA
>Date: Sun, 10 Mar 1996 11:15:33 +0100

>In article <Pine.SGI.3.91.960309100207.26744B-100000 at cypress.csc.fi>,
>"Markku.M Jeltsch" <jeltsch at csc.fi> wrote:

>> Occasionally when I digest genomic DNA (e.g. tail DNA from 
>> transgenic mice) I observe a distinct band in the pattern that looks 
>> otherwise completely digested. Does anyone know what is the reason of
>> this? 

>Repeated sequences

These are referred to as satellite DNA which constitutes a significant 
proportion of the mouse genome.  Similar bands can be seen in human DNA 
digests depending on the enzyme(s) used.




More information about the Methods mailing list