Taq stability?

Nicola Collie alocin at sanger.otago.ac.nz
Mon Mar 11 20:05:34 EST 1996


Christoph Ahlers (CAH at ifm.mh-hannover.de) wrote:
> In article <4hn278$ni1 at taco.cc.ncsu.edu>, Susan Jane Hogarth
> <sjhogart at unity.ncsu.edu> wrote:

> > Maybe this has been addressed before, but I've been wondering...
> > 
> > If Taq polymerase is so heat-stable that it can function after 40+
> rounds of 95
> > degrees, why are people so uptight about letting it get out of the freezer for
> > more than 3.5 seconds?
> > -- 

Maybe it's to make sure a communal stock is always in the freezer when
you want to use it?  Certainly beats traipsing around the lab peering
into everyone's ice-buckets... 

---
	Nicola Collie        
	alocin at sanger.otago.ac.nz 
	University of Otago, New Zealand



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