tips for blunt ended ligation!
MAD2631 at rsgis6.tamu.edu
Mon Mar 11 17:43:41 EST 1996
It is a good idea to dephophorylate vector ends because in most
expression vectors there is no blue white screening. Therefore your
chances of getting background is less. Otherwise you may have to screen
lots of colonies before you find one with insert. Even then your
chances of getting the insert in right orientation is 50% (remember your
insert can go in either orientation in blunt end ligations or with ends
of single restriction enzyme) . So good luck.
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