SEAP

[ole morten Seternes]

We have just bought the the SEAP reporter system from Clontech. We are
currently using CAT-assays in our transient transfection studies, but the
Secreted alkaline phosphatase system loocked much easier. But we are
unable to detect any  SEAP activety after transfection of NIH-3T3 cells.
We have used both calsium phosphate mediated and lipofectamin mediated
transfection methods. I wonder if there are anybody that have used this
reporter gene system with success?


Ole Morten Seternes