tips for blunt ended ligation!

Kenneth Y. Ilio you at somehost.somedomain
Mon Mar 11 16:22:05 EST 1996

I am trying to make an expression vector by blunt ended ligation. The 
vector is cut with Eco RI and ends are subsequently blunted. Before using 
this vector for ligation, is it necessary to dephosphorylate the ends? 
Thanks in advance.


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