transformation problems

CAShug cashug at aol.com
Tue Mar 12 00:28:39 EST 1996


Hi 

I usually add 5ul (of a total volume of 35ul gel ligation mix) to 100ul
competent cells. Perhaps you're using too much? I usually shake at 37 for
at least a hour but no longer than 90min. 
Good luck,
Chris



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