Distinct bands in restriction dig. of genomic DNA

Eitan Rubin bcrubin at dapsas1.weizmann.ac.il
Tue Mar 12 11:03:46 EST 1996

In article <ae.37.000B93EE at dna.bio.warwick.ac.uk>,
ae at dna.bio.warwick.ac.uk wrote:

> In article <mantei-1003961115330001 at retrograde.ethz.ch>
mantei at neuro.biol.ethz.ch (Ned Mantei) writes:
> >From: mantei at neuro.biol.ethz.ch (Ned Mantei)
> >Subject: Re: Distinct bands in restriction dig. of genomic DNA
> >Date: Sun, 10 Mar 1996 11:15:33 +0100
> >In article <Pine.SGI.3.91.960309100207.26744B-100000 at cypress.csc.fi>,
> >"Markku.M Jeltsch" <jeltsch at csc.fi> wrote:
> >> Occasionally when I digest genomic DNA (e.g. tail DNA from 
> >> transgenic mice) I observe a distinct band in the pattern that looks 
> >> otherwise completely digested. Does anyone know what is the reason of
> >> this? 
> >Repeated sequences
> These are referred to as satellite DNA which constitutes a significant 
> proportion of the mouse genome.  Similar bands can be seen in human DNA 
> digests depending on the enzyme(s) used.

Such bands are also observed with plants DNA.


Eitan Rubin, Plant Genetics, Weizmann Inst. of Science
Phone: (972)-8-9342421
Email: bcrubin at dapsas1.weizmann.ac.il
                  Shalom Haverim

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