cDNA library kits-your choice

RMMMS at CUNYVM.CUNY.EDU RMMMS at CUNYVM.CUNY.EDU
Tue Mar 12 11:16:26 EST 1996


It's been several years, but we had great results with Stratagene's Uni-ZAP II.
A lot probably depends on the quality of the RNA.  Back then we used a CsCl
method to purify total RNA and then an oligo dT column to get the poly-A RNA.
Howevr, there are many kits now for getting the poly-A directly.  It would be
worthwhile to get peoples opinions on these.



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