Leaky Mini-ProteanII unit.

Riekeltje Koedood rkoedood at bio.bu.edu
Tue Mar 12 12:13:55 EST 1996

mdpjr at cc.newcastle.edu.au wrote:
: We've had the same problem with at least 6 different units.  None have ever failed to leak.  We
: 'solved' it simply by filling the lower buffer almost to capacity, so that it was the same level
: (almost) as the upper electrode buffer.  We simply ignore the 'capillary siphoning' effect you
: describe.  Our gels run fine.  Never bothered to complain to BioRad any further.

We do the same - some units leak, some don't. I prefer the non-leaking ones,
saves on buffer and easier to see the slots for loading. However, if they
leak, we just fill up the lower buffer. Gels run fine.


More information about the Methods mailing list