35S-RFLPs

Javier Quinteiro bnjquint at usc.es
Tue Mar 12 07:16:34 EST 1996


Hi,
I'm looking for a method to:
- Labelling by PCR a PCR product with 35SdATP.
- Electrophoresis of fragments from hot PCR products (20-200 pb) in a 
Mini-Protean II electrophoresis system from BioRad.
If someone is familiar with these topics, I would appreciate some 
information mainly about how much 35S in PCR.
Thanks in advance.  

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                      Javier Quinteiro
                   Email:bnjquint at usc.es   
                      Fax:81-596904               
          Dpt. of Biochemistry and Molecular Biology
-----------------------------------------------------------
Javier Quinteiro. 
Dpto. de Bioquimica y Biologia Molecular
Facultad de Biologia. Universidad de Santiago.
15706 Santiago de Compostela. Galicia. SPAIN 
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