Artifact in plasmid preps

Mark R. Miglarese miglaresemr at
Tue Mar 12 15:27:10 EST 1996

It sounds like you have what we call "the ghost band".  It comes and 
goes with minipreps, but we usually don't see it in lg. scale (2xCsCl) 
preps.  It's permanently denatured DNA and wont transform, cut or mess 
up your ligations.  Having said that, I still don't like it hanging 
around.  It's your wishes.

Mark R. Miglarese, Ph.D.
Cancer Research
Pfizer Central Research
Box 372                        
Eastern Point Road              
Groton, CT 06340
Phone: (860) 441-8478
FAX:   (860) 441-1290
Email: miglaresemr at

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