Wizard PCR purification from agarose gel

Tae Gwon Oh taegob at plaza.snu.ac.kr
Wed Mar 13 10:34:58 EST 1996

ruth e.w. walker wrote:
> I am unable to purify my PCR product from the gel using the Wizard kit.
> It works well when I purify directly from the PCR rxn. I use 2% agarose,
> which melts within 4 min at 65C in presence of the resin. Is that too
> long? Too short? What else could be wrong, given that it does work with
> the PCR rxn, done concurrently?

Did you use TAE buffer ? It's important. After heat in 65C, then cool to 
r.t. I can help you.
TaeGwon Oh    taegob at plaza.snu.ac.kr
              FAX 2-886-5802
              TEL 2-880-7874
              Dept. of Microbiology, College of Pharmacy
              Seoul National UNIV. Seoul, KOREA

More information about the Methods mailing list