Ribozymes

Bradley A. Arrick Bradley.A.Arrick at dartmouth.edu
Tue Mar 12 17:30:21 EST 1996


I am interested in the use of ribozymes expressed from eukaryotic
expression constructs, with the goal of suppressing the level of a
particular mRNA species following stable transfection of a mammalian
cell line. Are there good expression constructs out there?  Does the
ribozyme have to be excised to be functional, or can it remain as part
of a larger RNA species?  Hammerhead vs hairpin.
Thanks.



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