Leaky Mini-ProteanII unit.

George Rutherford gruther at bilbo.bio.purdue.edu
Tue Mar 12 16:41:12 EST 1996


In article <4i4bcj$pf9 at news.bu.edu>, rkoedood at bio.bu.edu (Riekeltje
Koedood) wrote:

> mdpjr at cc.newcastle.edu.au wrote:
> : We've had the same problem with at least 6 different units.  None have
ever failed to leak.  We
> : 'solved' it simply by filling the lower buffer almost to capacity, so
that it was the same level
> : (almost) as the upper electrode buffer.  We simply ignore the
'capillary siphoning' effect you
> : describe.  Our gels run fine.  Never bothered to complain to BioRad
any further.
> 
> We do the same - some units leak, some don't. I prefer the non-leaking ones,
> saves on buffer and easier to see the slots for loading. However, if they
> leak, we just fill up the lower buffer. Gels run fine.
> 
> Marieke

In my experience, these units won't leak as long as they are scrupulously
clean. If you don't scrub them pretty well right after a run, small bits
of dried acrylamide will foul up the seals causing leakage in subsequent
runs.

George







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