Sfi1 enzyme

tera tjstbc at sd.cts.com
Tue Mar 12 15:34:35 EST 1996


Hello,

	Our group has been having great difficulties ligating PCR products into 
general vectors following digestion with Sfi1 enzyme.  The enzyme 
linearizes the vector fine so the stock is O.K.  According to NEB folks 
we have more than enough bases overhanging to ensure efficient digestion 
and the non-palindromic sequence is compatible.  Has anyone experienced 
any similar problems with this enzyme?  Any ideas?  Thanks and have a 
groovy day.





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