Wizard PCR purification from agarose gel

A. John Watson watson_j at bms.com
Tue Mar 12 14:23:17 EST 1996


In article <4hq4om$or4 at news.missouri.edu>, "ruth e.w. walker"
<pharmrew at muccmail.missouri.edu> wrote:

> I am unable to purify my PCR product from the gel using the Wizard kit. 
> It works well when I purify directly from the PCR rxn. I use 2% agarose, 
> which melts within 4 min at 65C in presence of the resin. Is that too 
> long? Too short? What else could be wrong, given that it does work with 
> the PCR rxn, done concurrently?

I use QiaQuick from Qiagen.  It *usually* works, though yield is often
loweer than I'd wish.  Other than that...

Cheers,

AJW

----------------
John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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