Protocol for Re-using of Qiagen tips

Trevor Bezdek trevor at leland.stanford.edu
Wed Mar 13 15:37:23 EST 1996


I'm not sure because I don't have the protocol in front of me but doesn't
Quiagen only recommed 50 ml of overnight culture for a maxi prep in order
to not overload the column?  Also, if you are using TG1 it will increase
the problem.

In article <Do7KEJ.AIL at uns.bris.ac.uk>, harry.witchel at bristol.ac.uk wrote:

>         1)  Too much bacterial material in starting material (I solubilize a 
> 250 ml overnight culture of Terrific broth into 10 ml P1, then add 10 ml P2 
> and P3).

--
Trevor Bezdek
Stanford Medical School
Developmental Biology
<trevor at leland.stanford.edu>



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