MICROSEQUENCING N-BLOCKED PROTEINS

Andy Brauer brauer at ARIAD.COM
Wed Mar 13 13:25:07 EST 1996


MICROSEQUENCING N-BLOCKED PROTEINS can be done more quickly and efficiently
than traditional methods involving enzymatic cleavage/HPLC separation of
peptides. We have developed a method that can identify an N-blocked protein
using a single sequencer run and avoids the problems inherent in the
traditional HPLC recovery of peptides (e.g. over-representation of
contaminant peptides, inefficiency of enzyme cleavage on sub-microgram
amounts of protein, and aggregation of peptides from very hydrophobic
proteins). If you have an N-blocked protein and are interested in learning
more about our approach to this problem, please E-mail a fax number and we
will return a more lengthy discussion.
    Andy Brauer
    brauer at ariad.com



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