Re-post: PCR through GC-rich

MAN!!! scapuano at cbil.humgen.upenn.edu
Wed Mar 13 12:00:06 EST 1996


Sorry if this was answered already.. Guess I didn't check soon enough, and
my original post is gone.  Any tips for PCR through very GC-rich region
(over 80%).  Have tried Vent polymerase, formamide, glycerol, touchdown,
hot starts, etc.  Thanks!



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