PCR through GC-rich

sreesh ssreesha at coconut.wustl.edu
Thu Mar 14 18:11:48 EST 1996



                           ************************
			      SREESHA SRINIVASA
			      CELL BIOL. PHYYSIOL
			      WASHINGTION UNIV
			      ST.LOUIS, MO 63130
			      PH # 314-362-1662
                              email add: ssreesha at mulberry.wustl.edu
           				 psreesha at cellbio.wuslt.edu
*******************************************************************************
			    
			   


On Fri, 8 Mar 1996, MAN!!! wrote:

> Anyone have suggestions for PCR through VERY GC-rich regions (80% or
> more).  Have tried formamide, glycerol, etc.
	have you tried 5% DMSO (final conc)? Pure DMSO (mol bio grade 
from Sigma) has worked for me. 
good luck and lemme know if u succeed.

> 
> Thanks!
> 
> 



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