NdeI: cutting problems?? anyone else?? seems silly! (fwd)

daniel morris dmorris at sbsnov1.auckland.ac.nz
Thu Mar 14 18:10:25 EST 1996


Ditto.  Use lots of enzyme, overnight incubations preferably, add more for
good luck every now and then, use CLEAN DNA, and allow at least six
base-pairs of extra sequence when designing primers containing terminal
NdeI sites.

Its a bastard of an enzyme.



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