Free Sulphydryl detection

Michael Daws Michael.Daws at ucmp.umu.se
Fri Mar 15 09:05:36 EST 1996


I have a 19-mer peptide (2200 MW) with a C-terminal cysteine which I 
purchased and received as a lyophilised powder.  The company told me 
that the peptide would be greater than 95% monomer, however the results 
I have been getting would suggest that the majority of the peptide is 
disulphide bonded.  Is there any nice easy colorimetric reagent I can 
use to detect free sulphydryl groups so that I can determine just how 
much of my peptide is disulphide bonded?  Alternatively does anybody 
know a recipe for a gel that I could use to detect such a difference? 
Thanks.

Mike Daws





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