Yolky DNA

vilimf01 at mcrcr6.med.nyu.edu vilimf01 at mcrcr6.med.nyu.edu
Fri Mar 15 02:02:04 EST 1996


Have you tried or considered CTAB precipitation? - just a thought


In article <Pine.A32.3.91.960314153414.111271A-100000 at aix1.uottawa.ca>, s060965 at AIX1.UOTTAWA.CA writes:
> Hello,
> 
> I'm trying to extract DNA from very early zebrafish embryos (blastula 
> stages and earlier).  I do the standard Prot. K, SDS, EDTA buffer 
> digestion ... followed by several phenol extractions, two 
> phenol/chloroform, and a final chloroform extraction.
> 
> When I go to precipitate the DNA using NaAce and isopropanol, yolk 
> precipitates out of the aqueous extraction.  A mess!!!!
> 
> Obviously, this yolk is water soluable, not extracted by the phenols or  
> disturbed by the Prot K.
> 
> Does anyone have any suggestions on how to get rid of this yolk, or a 
> better method of purifying DNA from early fish embryos.
> 
> Any help is very much appreciated.
> 
> Cristofre Martin
> Loeb Institute
> Ottawa Civic Hospital
> Ottawa, Ontario



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