cracked gels?

Eddie Diehl diehl001 at mc.duke.edu
Fri Mar 15 09:34:52 EST 1996


In article <4i96lu$ijs at news.doit.wisc.edu>, Helkor at facstaff.wisc.edu
(Helen Korsmo) wrote:

> Does anyone have a tried and true method for drying high percentage SDS
gels (>15%) without 
> cracks?  I'm using minigels, 1.5mm thick.

One of the things that makes gels crack upon drying is the acetic acid
present. Equilibrate your gel in distilled water before drying. If they
still crack, add 10% glycerol to the water, but obviously don't dry on a
gel dryer - use some of that special clear film that's available.

Good luck!

Eddie Diehl



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