Membrane properties

Achim Recktenwald achim at cam.org
Fri Mar 15 20:32:09 EST 1996


Michail P. Sahsamanoglou wrote:
> 
> Hi Netters.
> These are my questions: Are PVDF membranes exclusively used for protein
> immunoblots and microsequencing or can they be used also for nucleic
> acid blotting? What are the differences regarding blot applications
> between NC, nylon and PVDF and what is the consensus for their use?
> I will appreciate all your answers. TIA
> Mike


I can answer a small part of your question.
The "specialty" of PVDS-membranes is their extreme hydrophobicity. The cores 
of most if not all proteins are very hydrophobic. Since exposure to SDS 
denatures proteins, they tend to stick very well to hydrophobic surfaces 
during blotting.

Achim



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