Free Sulphydryl detection

Keld Sorensen KeldS at uic.edu
Fri Mar 15 18:04:37 EST 1996


[snip problems with dimerization of peptide]

peptides with a CYS tend to dimerize even when
lyophilized.
Detect the free CYS with Ellmans reagent.
If dimerized and the peptide too small for dialysis
(and thus DTT treatment) you could try the 
Reduce-Imm column from Pierce - it is fundamentally
an immobilized DTT on sepharose.

Keld.





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