PCR HELP! DXS52, Xq28 (St14 VNTR) anybody with experience?
Dr G R Taylor
ai80 at dial.pipex.com
Sat Mar 16 05:25:37 EST 1996
We use St14 sometimes, and it can be difficult. The basic
Richards et al conditions and low cycle numbers work on our MJ PTH60 and
Mini cyclers, although we use less DNA (25-50ng per 20ul reaction).
High cycle numbers result in loss of the larger alleles. We always hot
start, and sometimes cut the products with TaqI to reveal the internal
Hope this helps
gtaylor at hgmp.mrc.ac.uk
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