PCR HELP! DXS52, Xq28 (St14 VNTR) anybody with experience?

Dr G R Taylor ai80 at dial.pipex.com
Sat Mar 16 05:25:37 EST 1996


We use St14 sometimes, and it can be difficult. The basic 
Richards et al conditions and low cycle numbers work on our MJ PTH60 and 
Mini cyclers,  although we use less DNA (25-50ng per 20ul reaction).  
High cycle numbers result in loss of the larger alleles. We always hot 
start,  and sometimes cut the products with TaqI to reveal the internal 
RFLP.


Hope this helps

Graham Taylor
Leeds, UK
gtaylor at hgmp.mrc.ac.uk





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