Re-post: PCR through GC-rich

Dale Levitzke Dlevitzke at medicine.newcastle.edu.au
Sun Mar 17 23:50:36 EST 1996


Try some of the following,

Exo-pfu polymerase from intergrated sciences.
10-15% DMSO
1:1 ratio of 7-deaza dGTP to dGTP
denaturation temp 98 (1min) degrees followed by anneal and extension 
step at around 65 t0 68 degrees (3 min) for 30-35 cycles.





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