longggg mRNA transcript

Konstantin M. Chumakov chumakov at helix.nih.gov
Mon Mar 18 14:58:35 EST 1996

In Article <4icsdb$kv4 at hydra.acs.ttu.edu>, knben at ttacs.ttu.edu (benan
dincturk) wrote:
>        I am trying to clone a 5.6 kb gene from spinach. I screened a 
>cDNA library but no luck. Now I am trying to purify mRNA from fresh 
>tissue. Is there anybody who have suggestions to get longer transcripts?
> Benan

Try Gibco/BRL Superscript-2 RTase.  I routinely make 7.5k transcripts with
outstanding yield.

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