TNT kit with T7 RNA Pol

HONG u47888 at UIC.EDU
Mon Mar 18 21:36:45 EST 1996

Has anyone figured out how to get consistent results with T7 RNA pol. 
using promega TNT kit?  We get good results with sp6 RNA pol, but not 
with T7 RNA pol.  DNA templates used in T7 were linearized according 
to the promega protocol.

Thanks for any input.


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