phage display using Stratagene's Surfzap Kit

[tera]

Alex, We have been using this system regularly and, on average, obtain 
primary libraries in the 10E7 range.  We use either Gigapack Gold III 
extract or Epicentre extract (the latter is a lot cheaper and has 
comparable efficiencies). We habitually digest with Not/Spe overnight 
since Not1 is not the most efficient enzyme in the world.  Do you have a 
long (8+ bases) overhang on the Not1 end?  We also tend to ligate 
overnight at 4oC. Our background seems to vary between 10E2 and 10E3.  
Hope some of this is useful. Barry.