Blocked Xba I sites?

Todd Miller - Pharmacology tmiller at newssun.med.miami.edu
Tue Mar 19 08:56:45 EST 1996


I'm trying to characterize some mouse genomic clones and
one of the enzymes I've been using is Xba I.  I know that
some Xba I sites are blocked by methylation if the DNA
is maintained in bacteria that are not dam(-).  But are Xba I
sites ever blocked in eukaryotic DNA?  

Thanks in advance.

Todd Miller
tmiller at newssun.med.miami.edu

 



More information about the Methods mailing list