RNA isolation/Chomozynski

Daniel Pereira mgs6145 at student.health.ufl.edu
Tue Mar 19 17:19:39 EST 1996


Susan Patrick <patrics at okstate.edu> wrote:
>
> We are using a modification of the Chomczynski single-step method of RNA 
> isolation.  We have n-lauroylsarcosine and the sodium salt.  Our 
> solution calls for 0.5% sarcosyl.  Is this needed in percent weight or 
> by volume?
> 
> 
I use as % weight-never had any problems. Best of Luck.




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