longggg mRNA transcript

Bob Horton horto005 at maroon.tc.umn.edu
Tue Mar 19 09:17:11 EST 1996

Konstantin M. Chumakov wrote:
> In Article <4icsdb$kv4 at hydra.acs.ttu.edu>, knben at ttacs.ttu.edu (benan
> dincturk) wrote:
> >        I am trying to clone a 5.6 kb gene from spinach. I screened a
> >cDNA library but no luck. Now I am trying to purify mRNA from fresh
> >tissue. Is there anybody who have suggestions to get longer transcripts?
> Try Gibco/BRL Superscript-2 RTase.  I routinely make 7.5k transcripts with
> outstanding yield.

Just curious - do you use random primers, or oligo dT?

Robert M. Horton                                       Have a :) day

"Scotty, try flushing the radioactive waste into the ventilation 

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