Expression vector tags

John Elliott johne at gdpiris.chem.sunysb.edu
Tue Mar 19 23:09:46 EST 1996


In article <96079.132118RMMMS at CUNYVM.CUNY.EDU>, <RMMMS at CUNYVM.CUNY.EDU> wrote:

> The StrepTag system of Shmidt and Skerra, Prot. Engineering 6:109-122, 1985
> uses a carboxy terminal tag for purification on strepavidin agarose columns.
> It is available commercially, but I forget from whom.  I haven't used it, but
> it seems like what you are looking for.
> Ron Magnusson

I'm not sure, but the Promega Pinpoint vector has some kind of biotinylated
fusion tag that can be used to purify on streptavidin.  But it is N-terminal
fusion.
jte



More information about the Methods mailing list