Expression vector tags
johne at gdpiris.chem.sunysb.edu
Tue Mar 19 23:14:42 EST 1996
In article <J.L.Hobman.75.0010995E at bham.ac.uk>, J.L.Hobman at bham.ac.uk (Jon
> Hi Folks,
> Does anyone know of an expression vector that has an affinity tag for the
> C-terminal end of the protein that they want to overexpress that is not 6XHis?
> I don't have a problem finding N-terminal tagged vectors but I think
> would seriously interfere with the DNA-binding ability of my protein.
> Thanks for your time,
> Jon Hobman
This isn't going to be much help, but I some researchers made C- and N-terminal
GST constructs that worked to purify fusion proteins. The reference is
Gene 162, 235-7. Maybe you can write to them ?
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