Expression vector tags

John Elliott johne at gdpiris.chem.sunysb.edu
Tue Mar 19 23:14:42 EST 1996


In article <J.L.Hobman.75.0010995E at bham.ac.uk>, J.L.Hobman at bham.ac.uk (Jon
Hobman) wrote:

> Hi Folks,
> 
> Does anyone know of an expression vector that has an affinity tag for the 
> C-terminal end of the protein that they want to overexpress that is not 6XHis?
> I don't have a problem finding N-terminal tagged vectors but I think
that this 
> would seriously interfere with the DNA-binding ability of my protein.
> 
> Thanks for your time,
> 
> Jon Hobman

This isn't going to be much help, but I some researchers made C- and N-terminal
GST constructs that worked to purify fusion proteins.  The reference is
Gene 162, 235-7.  Maybe you can write to them ?

jte.



More information about the Methods mailing list