A260 or Fluoromete ??? Which is correct
john.brennand at gbapr.zeneca.com
Wed Mar 20 08:07:08 EST 1996
Yep it does (but less efficiently). However, I think most of the
absorbtion at 260 in this case is coming from very small RNA fragments
and in particular from single nucleosides/nucleotides. These do not
bind EtBr very well and hence don't fluoresce well at all, either in
solution or on a gel.
Hence the discrepancies between the methods, and as you say, with
fluorescence in solution probably being the most accurate.
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