A260 or Fluoromete ??? Which is correct

john brennand john.brennand at gbapr.zeneca.com
Wed Mar 20 08:07:08 EST 1996


Walt

Yep it does (but less efficiently). However, I think most of the 
absorbtion at 260 in this case is coming from very small RNA fragments 
and in particular from single nucleosides/nucleotides.  These do not 
bind EtBr very well and hence don't fluoresce well at all, either in 
solution or on a gel.

Hence the discrepancies between the methods, and as you say, with 
fluorescence in solution probably being the most accurate.

John





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