messy agarose bands

john brennand john.brennand at gbapr.zeneca.com
Wed Mar 20 07:04:56 EST 1996


One thing we know makes a big difference to the sharpness of bands in 
agarose gels (and acrylamide gels) is the composition of the loading 
buffer.

There are n recipes around for these using all sorts of "density 
enhancers" ficoll, glycerol, DMSO etc

Years ago we tried 'em all, head to head, and by far the best in our 
hands was sucrose.  We make a 40% sucrose / 200mM EDTA/ bromophenol 
blue, stock (store at 4oC for years!) and add 1/10 V/V to restriction 
digests, fragments, etc.

Try it and see if that helps.

John





More information about the Methods mailing list