messy agarose bands
john.brennand at gbapr.zeneca.com
Wed Mar 20 07:04:56 EST 1996
One thing we know makes a big difference to the sharpness of bands in
agarose gels (and acrylamide gels) is the composition of the loading
There are n recipes around for these using all sorts of "density
enhancers" ficoll, glycerol, DMSO etc
Years ago we tried 'em all, head to head, and by far the best in our
hands was sucrose. We make a 40% sucrose / 200mM EDTA/ bromophenol
blue, stock (store at 4oC for years!) and add 1/10 V/V to restriction
digests, fragments, etc.
Try it and see if that helps.
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