cracked gels?
Dr E. Buxbaum
EB15 at le.ac.uk
Mon Mar 18 12:04:05 EST 1996
Fix, stain and destain the gel as with lower percentage gels. Then
incubate them with 2 changes of distilled water and finally 5% glycerol.
Put the gel between 2 sheets of cellophane into a drying frame (can be
bought at ridiculous price from a lab supplier or cut cheaply from a
sheet of plastic) and air dry over night. This leaves you with a clear,
crack free gel, which can be photographed, projected with an overhead or
stored in a lab book. Should you ever wish, you can cut out a band with
a razor blade, rehydrate in water (the cellophane comes of at this stage)
and use for electroelution, immunisation ect., even years after you made
the gel!
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