Primer extention analysis by PCR

Ken Howe howe at DARWIN.UCSC.EDU
Wed Mar 20 19:53:35 EST 1996


Toshio,
The 5' end of any product in your RT-PCR reaction will be defined by your 
oligo, so RT-PCR cannot be used to map the 5' end of an RNA (I'm assuming 
you want the transcriptional start site?).

Ken

On Wed, 20 Mar 1996 
Toshio.SAKAMOTO at net.bio.net wrote:

> Date: Wed, 20 Mar 1996 15:19:01 EST
> From: Toshio.SAKAMOTO at net.bio.net
> To: methods at net.bio.net
> Subject: Primer extention analysis by PCR
> 
> I am wondering if someone try to determined the 5' end of mRNA by
> RT-PCR.  My transcripts might be small amount in RNA fraction, thus
> if the 1st synthesized cDNA can be amplified by RT-PCR, it's very helpful.
> Any information will be appreciated.  Thanks.
> 
> Toshio Sakamoto
> txs25 at psuvm.psu.edu
> 
> 
> 



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