small PCR product-->ligation

[luis]

Hi !!!!

			 I have a small PCR product (300 base pairs) afther the cut with
KpnI and AflII, that need to be inserted in a plasmid. Is the first time 
that I do a ligation (T4 DNA ligase) and I have doubts if the size of 
the insert may influence the efficience of the reaction. Could anybody  
give me advices for improve the reaccion? 
											

																																								
																																									Thanks in advance

																																																Luis