small PCR product-->ligation

luis lgbrieba at academ01.mty.itesm.mx
Wed Mar 20 21:18:17 EST 1996


Hi !!!!

			 I have a small PCR product (300 base pairs) afther the cut with
KpnI and AflII, that need to be inserted in a plasmid. Is the first time 
that I do a ligation (T4 DNA ligase) and I have doubts if the size of 
the insert may influence the efficience of the reaction. Could anybody  
give me advices for improve the reaccion? 
											

																																								
																																									Thanks in advance

																																																Luis





More information about the Methods mailing list