small PCR product-->ligation

luis lgbrieba at
Wed Mar 20 21:18:17 EST 1996

Hi !!!!

			 I have a small PCR product (300 base pairs) afther the cut with
KpnI and AflII, that need to be inserted in a plasmid. Is the first time 
that I do a ligation (T4 DNA ligase) and I have doubts if the size of 
the insert may influence the efficience of the reaction. Could anybody  
give me advices for improve the reaccion? 

																																									Thanks in advance


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