Modified pGEM?

Angel Yanagihara angel at
Thu Mar 21 21:17:48 EST 1996

Has anyone out there had any experience at knocking out restriction
sites(not in the polycloning region) in vectors in order to get
the site unique in your insert?  My problem is that I am working
with an insert that has a Sap I site which would be very useful
to me if i could get it out of my vector (pGEM).  Any handy 
approaches or recommendations for other constructs that might be
useful would be appreciated.


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