Expression vector tags

Kiley R. Prilliman kprillim at rex.re.uokhsc.edu
Fri Mar 22 08:30:47 EST 1996


Try the FLAG system from Kodak...you make primers to introduce the 
FLAG epitope (it's not too big, only about 8 aa's) at either the 
N-terminus, C-terminus, or internally (rather wide variety of 
options!), and Kodak has antidodies to each of these epitope 
situations.  Alternatively, you can also purchase from the gel to 
which the Ab of choice is already coupled, and purification from there 
is a snap!



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